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Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis

  • Jin Woo BokEmail author
  • Nancy P. Keller
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 944)

Abstract

Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Here we introduce a modified Quick-change site-directed mutagenesis method that allows for rapid and accurate construction of fungal transformation vectors.

Key words

Plasmid vector Restriction enzyme Polymerase chain reaction Quick-change site-directed mutagenesis 

Notes

Acknowledgment

The project was supported by PO1GM084077 from the National Institute of General Medical Sciences to N.P.K.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Medical Microbiology and ImmunologyUniversity of Wisconsin-MadisonMadisonUSA

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