Advertisement

Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy

  • Verena RakerEmail author
  • Joachim Maxeiner
  • Angelika B. Reske-Kunz
  • Stephan Sudowe
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 940)

Abstract

Gene gun-mediated delivery of allergen-encoding plasmid DNA has been in focus for many years now as being a needle-free alternative to the protein-based desensitization regimen used in specific immunotherapy. Biolistic immunization with the Helios gene gun has proven to be potent in the induction of antigen-specific CD4+ and CD8+ T cells. Here we describe biolistic vaccination in experimental mouse models of IgE-mediated type I allergy as well as allergen-induced airway inflammation.

Key words

Allergy DNA vaccination Gene gun IgE Allergic airway inflammation 

References

  1. 1.
    Raz E et al (1996) Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization. Proc Natl Acad Sci USA 93:5141–5145PubMedCrossRefGoogle Scholar
  2. 2.
    Chua KY, Kuo IC, Huang CH (2009) DNA vaccines for the prevention and treatment of allergy. Curr Opin Allergy Clin Immunol 9:50–54PubMedCrossRefGoogle Scholar
  3. 3.
    Fynan EF et al (1993) DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc Natl Acad Sci USA 90:11478–11482PubMedCrossRefGoogle Scholar
  4. 4.
    Pertmer TM et al (1995) Gene gun-based nucleic acid immunization: elicitation of humoral and cytotoxic T lymphocyte responses following epidermal delivery of nanogram quantities of DNA. Vaccine 13:1427–1430PubMedCrossRefGoogle Scholar
  5. 5.
    Sudowe S et al (2003) Transcriptional targeting of dendritic cells in gene gun-mediated DNA immunization favors the induction of type 1 immune responses. Mol Ther 8:567–575PubMedCrossRefGoogle Scholar
  6. 6.
    Sudowe S et al (2009) Uptake and presentation of exogenous antigen and presentation of endogenously produced antigen by skin dendritic cells represent equivalent pathways for the priming of cellular immune responses following biolistic DNA immunization. Immunology 128:e193–e205PubMedCrossRefGoogle Scholar
  7. 7.
    Pertmer TM, Roberts TR, Haynes JR (1996) Influenza virus nucleoprotein- specific immunoglobulin G subclass and cytokine responses elicited by DNA vaccination are dependent on the route of vector DNA delivery. J Virol 70:6119–6125PubMedGoogle Scholar
  8. 8.
    Yoshida A et al (2000) Advantage of gene gun-mediated over intramuscular inoculation of plasmid DNA vaccine in reproducible induction of specific immune responses. Vaccine 18:1725–1729PubMedCrossRefGoogle Scholar
  9. 9.
    Ross R et al (2003) Transcriptional targeting of dendritic cells for gene therapy using the promoter of the cytoskeletal protein fascin. Gene Ther 10:1035–1040PubMedCrossRefGoogle Scholar
  10. 10.
    Ludwig-Portugall I et al (2004) Prevention of long-term IgE antibody production by gene gun-mediated DNA vaccination. J Allergy Clin Immunol 114:951–957PubMedCrossRefGoogle Scholar
  11. 11.
    Sudowe S et al (2006) Prophylactic and therapeutic intervention in IgE responses by biolistic DNA vaccination primarily targeting dendritic cells. J Allergy Clin Immunol 117:196–203PubMedCrossRefGoogle Scholar
  12. 12.
    Zindler E et al (2008) Divergent effects of biolistic gene transfer in a mouse model of allergic airway inflammation. Am J Respir Cell Mol Biol 38:38–46PubMedCrossRefGoogle Scholar
  13. 13.
    Baniyash M, Eshhar Z (1984) Inhibition of IgE binding to mast cells and basophils by monoclonal antibodies to murine IgE. Eur J Immunol 14:799–807PubMedCrossRefGoogle Scholar
  14. 14.
    Conrad ML et al (2009) Comparison of adjuvant and adjuvant-free murine experimental asthma models. Clin Exp Allergy 39:1246–1254PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Verena Raker
    • 1
    Email author
  • Joachim Maxeiner
    • 2
  • Angelika B. Reske-Kunz
    • 3
  • Stephan Sudowe
    • 4
  1. 1.Clinical Research Unit Allergology, Department of DermatologyUniversity Medical Center of the Johannes Gutenberg University MainzMainzGermany
  2. 2.Asthma Core Facility, Research Center ImmunologyUniversity Medical Center of the Johannes Gutenberg University MainzMainzGermany
  3. 3.Department of DermatologyUniversity Medical Center of the Johannes Gutenberg-UniversityMainzGermany
  4. 4.Ganzimmun Diagnostics AGMainzGermany

Personalised recommendations