Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy

  • Verena RakerEmail author
  • Joachim Maxeiner
  • Angelika B. Reske-Kunz
  • Stephan Sudowe
Part of the Methods in Molecular Biology book series (MIMB, volume 940)


Gene gun-mediated delivery of allergen-encoding plasmid DNA has been in focus for many years now as being a needle-free alternative to the protein-based desensitization regimen used in specific immunotherapy. Biolistic immunization with the Helios gene gun has proven to be potent in the induction of antigen-specific CD4+ and CD8+ T cells. Here we describe biolistic vaccination in experimental mouse models of IgE-mediated type I allergy as well as allergen-induced airway inflammation.

Key words

Allergy DNA vaccination Gene gun IgE Allergic airway inflammation 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Verena Raker
    • 1
    Email author
  • Joachim Maxeiner
    • 2
  • Angelika B. Reske-Kunz
    • 3
  • Stephan Sudowe
    • 4
  1. 1.Clinical Research Unit Allergology, Department of DermatologyUniversity Medical Center of the Johannes Gutenberg University MainzMainzGermany
  2. 2.Asthma Core Facility, Research Center ImmunologyUniversity Medical Center of the Johannes Gutenberg University MainzMainzGermany
  3. 3.Department of DermatologyUniversity Medical Center of the Johannes Gutenberg-UniversityMainzGermany
  4. 4.Ganzimmun Diagnostics AGMainzGermany

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