Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy
Gene gun-mediated delivery of allergen-encoding plasmid DNA has been in focus for many years now as being a needle-free alternative to the protein-based desensitization regimen used in specific immunotherapy. Biolistic immunization with the Helios gene gun has proven to be potent in the induction of antigen-specific CD4+ and CD8+ T cells. Here we describe biolistic vaccination in experimental mouse models of IgE-mediated type I allergy as well as allergen-induced airway inflammation.
Key wordsAllergy DNA vaccination Gene gun IgE Allergic airway inflammation
- 6.Sudowe S et al (2009) Uptake and presentation of exogenous antigen and presentation of endogenously produced antigen by skin dendritic cells represent equivalent pathways for the priming of cellular immune responses following biolistic DNA immunization. Immunology 128:e193–e205PubMedCrossRefGoogle Scholar