Protocol

Biolistic DNA Delivery

Volume 940 of the series Methods in Molecular Biology pp 305-315

Date:

Biolistic DNA Vaccination Against Trypanosoma Infection

  • Marianne BryanAffiliated withDepartment of Immunology, University of Pittsburgh, School of MedicineDepartment of Immunology, University of Washington
  • , Siobhan GuyachAffiliated withDepartment of Immunology, University of Pittsburgh, School of Medicine
  • , Karen A. NorrisAffiliated withDepartment of Immunology, University of Pittsburgh, School of Medicine Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Immunization to protect against Trypanosoma cruzi infection has the potential to greatly decrease the burden of Chagas’ disease in the Americas. Several target antigens have been explored by multiple investigators and show promise, but given that this parasite has multiple stages within the mammalian host, with both intracellular and extracellular forms, a multivalent vaccine will probably be necessary to provide complete immunity and prevent disease. Therefore, DNA immunization is an attractive method for efficient and effective delivery of multiple target antigens. In addition, the target population for a T. cruzi vaccine lives predominately in poorer rural areas in South America, making the stable DNA-gold precipitate, which does not require a cold-chain, used in biolistic immunization an attractive method for vaccination. Here we describe a biolistic immunization protocol that is capable of generating high titer antibody responses to recombinant T. cruzi vaccine targets and the in vitro preparation of T. cruzi for use in experimental vaccine challenge studies.

Key words

Trypanosoma cruzi DNA vaccination Chagas’ disease Parasite Infection