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Expression Pattern Analysis of MicroRNAs in Caenorhabditis elegans

  • Meltem Isik
  • Eugene BerezikovEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 936)

Abstract

MicroRNAs (miRNAs) are ∼22 nucleotide single-stranded RNA molecules that originate from hairpin precursors and regulate gene expression at the posttranscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. miRNAs play important roles in a variety of biological processes, including development, proliferation, differentiation, cell fate determination, apoptosis, signal transduction, host–viral interactions, and tumorigenesis. Methodological advances in miRNA studies allowed identification of biological roles for many miRNAs, and establishing the spatiotemporal expression patterns of miRNAs is one of the approaches to elucidate their biological functions. Expression pattern analysis of miRNAs helps to identify potential genetic interactors that exhibit similar expression patterns and this, combined with further supporting experiments, helps to identify the genetic pathways in which the specific miRNAs are involved. In this chapter, we describe a detailed protocol for the analysis of miRNA expression patterns in Caenorhabditis elegans.

Key words

microRNA Caenorhabditis elegans Expression patterns Functional analysis of miRNAs 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Hubrecht Institute, Royal Netherlands Academy of Arts and SciencesUniversity Medical Center UtrechtUtrechtThe Netherlands

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