Retinal Degeneration pp 215-226
High-Throughput RNA In Situ Hybridization in Mouse Retina
The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.
Key wordsRNA Gene expression Cellular resolution Hybridization Digoxigenin Riboprobe Chromogenic Retina Photoreceptor Development