Retinal Degeneration pp 215-226

Part of the Methods in Molecular Biology book series (MIMB, volume 935)

High-Throughput RNA In Situ Hybridization in Mouse Retina

Protocol

Abstract

The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.

Key words

RNA Gene expression Cellular resolution Hybridization Digoxigenin Riboprobe Chromogenic Retina Photoreceptor Development 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.The Salomon H. Snyder Department of NeuroscienceJohn Hopkins University School of MedicineBaltimoreUSA

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