Animal Models in Diabetes Research pp 31-44

Part of the Methods in Molecular Biology book series (MIMB, volume 933)

The BB Rat as a Model of Human Type 1 Diabetes

Protocol

Abstract

The BB rat is an important rodent model of human type 1 diabetes (T1D) and has been used to study mechanisms of diabetes pathogenesis as well as to investigate potential intervention therapies for clinical trials. The Diabetes-Prone BB (BBDP) rat spontaneously develops autoimmune T1D between 50 and 90 days of age. The Diabetes-Resistant BB (BBDR) rat has similar diabetes-susceptible genes as the BBDP, but does not become diabetic in viral antibody-free conditions. However, the BBDR rat can be induced to develop T1D in response to certain treatments such as regulatory T cell (Treg) depletion, toll-like receptor ligation, or virus infection. These diabetes-inducible rats develop hyperglycemia under well-controlled circumstances and within a short, predictable time frame (14–21 days), thus facilitating their utility for investigations of specific stages of diabetes development. Therefore, these rat strains are invaluable models for studying autoimmune diabetes and the role of environmental factors in its development, of particular importance due to the influx of studies associating virus infection and human T1D.

Key words

BB rat Type 1 diabetes Regulatory T cell Toll-like receptor Virus infection Major histocompatibility complex Lymphopenia Insulitis Kilham rat virus Enterovirus Innate immunity 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Program in Molecular MedicineUniversity of Massachusetts Medical SchoolWorcesterUSA

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