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Light Sheet Microscopy in Cell Biology

  • Raju Tomer
  • Khaled Khairy
  • Philipp J. KellerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 931)

Abstract

Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications.

Here, we provide an overview of light sheet-based microscopy assays for in vitro and in vivo imaging of biological samples, including cell extracts, soft gels, and large multicellular organisms. We furthermore describe computational tools for basic image processing and data inspection.

Key words

Light sheet microscopy In vivo imaging Cell extracts Quantitative developmental biology Embryonic development Image processing 

Notes

Acknowledgement

This work was supported by the Howard Hughes Medical Institute.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Janelia Farm Research Campus, Howard Hughes Medical InstituteAshburnUSA

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