Glycosylation research has gained significant attention in several research fields including immunology, protein production, and biomarker discovery. However, complex and time-consuming protocols are often necessary to obtain suitable samples for analysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans from serum samples. It only involves the subsequent addition of reagents and incubation in a PCR thermocycler. Moreover, this assay allows the detection of these glycans, which are only present in minute amounts in serum, on high-throughput microfluidics CE platforms including the MCE-202 MultiNA, 2100 Bioanalyzer, and eGene system. Using this clinical glycomics assay, we could reliably measure GlycoHepatoTest, a panel of biomarkers allowing the follow-up of chronic liver disease patients from the early stage onward.
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Research was supported by a GOA grant (BOFF07/GOA/017) from Ghent University, by Marie Curie Excellence Grant No. 014292 (EU-Framework Programme 6) and by the Fondation Fournier-Majoie pour l’Innovation (FFMI). Dr. D. Vanderschaeghe is a postdoctoral fellow of FWO (Fonds Wetenschappelijk Onderzoek—Vlaanderen).
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