Malaria pp 35-49 | Cite as

Ex Vivo Culture of Plasmodium Vivax and Plasmodium Cynomolgi and In Vitro Culture of Plasmodium Knowlesi Blood Stages

  • Anne-Marie Zeeman
  • Annemarie Voorberg-van der Wel
  • Clemens H. M. KockenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 923)


Long-term in vitro cultures of blood-stage parasites are so far feasible only for Plasmodium falciparum and P. knowlesi. In this chapter, we describe short-term ex vivo culturing of P. cynomolgi and P. vivax. We also describe long-term in vitro culturing of P. knowlesi as well as some techniques for synchronizing parasites. Cultured parasites can be used for a variety of purposes, e.g., for in vitro drug assays and antibody-mediated growth inhibition assays.

Key words

Plasmodium vivax Plasmodium cynomolgi Plasmodium knowlesi In vitro culture Drug assay Cryopreservation Rhesus Macaca mulatta Parasite synchronization 



The authors wish to thank Hasting Ozwara Suba for setting up the long-term in vitro culture of P. knowlesi, Joyce Verburgh for help in the in vitro drug assays, Els Klooster for providing parasite pictures, and Henk van Westbroek for designing the figures.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Anne-Marie Zeeman
    • 1
  • Annemarie Voorberg-van der Wel
    • 1
  • Clemens H. M. Kocken
    • 1
    Email author
  1. 1.Biomedical Primate Research CentreRijswijkThe Netherlands

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