Malaria pp 385-400 | Cite as

Quantification of Sporozoite Invasion, Migration, and Development by Microscopy and Flow Cytometry

  • Photini SinnisEmail author
  • Patricia De La Vega
  • Alida Coppi
  • Urszula Krzych
  • Maria M. Mota
Part of the Methods in Molecular Biology book series (MIMB, volume 923)


There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.

Key words

Invasion Attachment Cell traversal Exoerythrocytic stages Sporozoites Malaria Plasmodium Flow cytometry 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Photini Sinnis
    • 1
    Email author
  • Patricia De La Vega
    • 2
  • Alida Coppi
    • 3
  • Urszula Krzych
    • 2
  • Maria M. Mota
    • 4
  1. 1.Department of Molecular Microbiology and ImmunologyJohn Hopkins Bloomberg School of Public HealthBaltimoreUSA
  2. 2.Department of Cellular ImmunologyWalter Reed Army Institute of ResearchSilver SpringUSA
  3. 3.Regeneron PharmaceuticalsTarrytownUSA
  4. 4.Malaria UnitInstituto de Medicina MolecularLisboaPortugal

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