Membrane Protein Structure and Dynamics pp 17-24 | Cite as
Vapor Diffusion-Controlled Meso Crystallization of Membrane Proteins
Protocol
First Online:
Abstract
The presented method to crystallize membrane proteins combines the advantages of the meso-phase crystallization method and the classical vapor diffusion crystallization. It allows fast screening of crystallization conditions employing automated liquid handlers suited for the 96-well crystallization format.
Key words
Membrane protein crystallization In meso crystallization Lipidic cubic phase Meso phase Vapor diffusion MonooleinReferences
- 1.Landau EM, Rosenbusch JP (1996) Lipidic cubic phases: a novel concept for the crystallization of membrane proteins. Proc Natl Acad Sci U S A 93:14532–14535PubMedCrossRefGoogle Scholar
- 2.Qiu H, Caffrey M (2000) The phase diagram of the monoolein/water system: metastability and equilibrium aspects. Biomaterials 21:223–234PubMedCrossRefGoogle Scholar
- 3.Benvenuti M, Mangani S (2007) Crystallization of soluble proteins in vapour diffusion for X-ray crystallography. Nat Protoc 2:1633–1651PubMedCrossRefGoogle Scholar
- 4.Wöhri AB et al (2008) A lipidic-sponge phase screen for membrane protein crystallization. Structure 16:1003–1009PubMedCrossRefGoogle Scholar
- 5.Caffrey M, Cherezov V (2009) Crystallizing membrane proteins using lipidic mesophases. Nat Protoc 4:706–731PubMedCrossRefGoogle Scholar
- 6.McPherson A, Cudney B (2006) Searching for silver bullets: an alternative strategy for crystallizing macromolecules. J Struct Biol 156:387–406PubMedCrossRefGoogle Scholar
- 7.Kors CA et al (2009) Effects of impurities on membrane-protein crystallization in different systems. Acta Crystallogr D 65:1062–1073PubMedCrossRefGoogle Scholar
- 8.Misquitta Y, Caffrey M (2003) Detergents destabilize the cubic phase of monoolein: implications for membrane protein crystallization. Biophys J 85:3084–3096PubMedCrossRefGoogle Scholar
- 9.Chayen NE (1997) A novel technique to control the rate of vapour diffusion, giving larger protein crystals. J Appl Crystallogr 30:198–202CrossRefGoogle Scholar
- 10.Li F, Robinson H, Yeung ES (2005) Automated high-throughput nanoliter scale protein crystallization screening. Anal Bioanal Chem 383:1034–1041PubMedCrossRefGoogle Scholar
- 11.Brumshtein B et al (2008) Control of the rate of evaporation in protein crystallization by the microbatch under oil method. J Appl Crystallogr 41:969–971PubMedCrossRefGoogle Scholar
Copyright information
© Springer Science+Business Media, LLC 2012