Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 925)

Abstract

Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing approach for unbiased enrichment of DNA that is methylated on cytosines. The principle is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody that specifically recognizes 5-methylcytidine (5mC), which can be combined with PCR or high-throughput analysis (microarrays, deep sequencing). The MeDIP technique has been originally used to generate DNA methylation profiles on a genome scale in mammals and plants. Here we provide an optimized version of the MeDIP protocol suitable for low amounts of DNA, which can be used to study DNA methylation in cellular populations available in small quantities.

Key words

DNA methylation MeDIP Cytosine CpG Profiling Epigenomics Microarrays Deep sequencing 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Julie Borgel
    • 1
  • Sylvain Guibert
    • 1
    • 2
  • Michael Weber
    • 1
    • 2
  1. 1.Institute of Molecular Genetics, UMR 5535Université Montpellier 2, Université Montpellier 1, CNRSMontpellierFrance
  2. 2.UMR 7242 Biotechnology and Cell SignallingUniversité de Strasbourg, CNRS, ESBSIllkirchFrance

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