Perspectives on Methodology for In Vitro Culture of Helicobacter pylori

  • Timothy L. Cover
Part of the Methods in Molecular Biology book series (MIMB, volume 921)


Over the past 25 years, a variety of methods have been developed for culture of Helicobacter pylori in vitro. H. pylori is a capnophilic and microaerophilic organism that is typically cultured using complex culture media. Analysis of H. pylori growth in chemically defined media has provided insight into the nutritional requirements, physiology, and metabolic capacities of this organism.

Key words

Helicobacter pylori Defined medium Capnophilic Microaerophilic Nutritional ­requirements Nutrient acquisition 



Supported by National Institutes of Health (R01 AI039657, R01 AI068009, P01 CA116087) and Department of Veterans Affairs.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Medicine and Department of Pathology, Microbiology and ImmunologyVanderbilt University School of MedicineNashvilleUSA

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