Leaf Hue Measurements: A High-Throughput Screening of Chlorophyll Content

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 918)

Abstract

Computer analysis of digital photographic images provides fast, high-throughput screening of leaf pigmentation. Pixel-by-pixel conversion of red, green, blue (RGB) parameters to hue, saturation, value (HSV) showed that Hue values were proportional to total chlorophyll, offering an alternative to photometric analysis of leaf extracts. This is demonstrated using tobacco leaves with various chlorophyll contents due to senescence but shows the possibility of applications in studies of stress conditions accompanied by chlorophyll loss.

Key words:

Chlorophyll fluorescence High-throughput screening Leaf Hue Photochemical yield RGB color model HSV color model 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institute of Plant PhysiologyBiological Research CentreSzegedHungary
  2. 2.Institute of Biology, Faculty of SciencesUniversity of PécsPécsHungary

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