High-Throughput Phenotyping in Plants pp 151-164 | Cite as
Highly Sensitive High-Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography–Tandem Mass Spectrometry
Abstract
We describe a method for highly sensitive high-throughput analysis of six major phytohormones; cytokinins (23 species), auxins (7 species), abscisic acid (ABA), gibberellins (GAs, 11 species), salicylic acid (SA), and jasmonic acid (JA). The method consists of solid-phase extraction using 96-well column plates and liquid chromatography coupled with a tandem quadrupole mass spectrometer. In order to improve the quantification limit of negatively charged compounds and especially GAs, we use a chemical modification with an “MS-probe” that contains a quaternary amine moiety. The method requires plant tissue of <100 mg fresh weight for quantification and enables us to analyze large number of plant samples at a time.
Key words:
Abscisic acid Auxins Cytokinins Gibberellins Jasmonic acid Mass spectrometry MS-probes Plant hormones Salicylic acid Solid-phase extractionReferences
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