Generating Diploid Embryos from Xenopus tropicalis

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 917)

Abstract

A spectacular advantage of Xenopus tropicalis is the ease with which diploid embryos can be generated year round. By the simple administration of human chorionic gonadotropin, an investigator can generate many hundreds of synchronized embryos by in vitro fertilization or thousands of embryos from a mating pair. The ability to induce ovulations when desired facilitates many different experiments such as experimental embryology, molecular manipulation of gene products, and genetics.

Key words

Xenopus tropicalis Egg Sperm Embryo In vitro fertilization hCG Cysteine 

References

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    Khokha MK et al (2002) Techniques and probes for the study of Xenopus tropicalis development. Dev Dyn 225:499–510PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Pediatrics and GeneticsYale University School of MedicineNew HavenUSA

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