Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Specific Responses to Salinity Stress in Arabidopsis and Rice
Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.
Key wordsFACS Cell type-specific Salinity Rice Arabidopsis Protoplast GAL4
This work was supported by grants from the Australian Research Council (DP0770966 to M.T. and A.A.T.J.) and the U.S. National Institutes of Health (NIH grant R01 GM078279 to K.D.B.)
- 9.Bargmann BOR, Birnbaum KD (2010) Fluorescence activated cell sorting of plant protoplasts. J Vis Exp (36):e1673Google Scholar