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Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Specific Responses to Salinity Stress in Arabidopsis and Rice

  • Aurelie Evrard
  • Bastiaan O. R. Bargmann
  • Kenneth D. Birnbaum
  • Mark Tester
  • Ute Baumann
  • Alexander A. T. Johnson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 913)

Abstract

Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.

Key words

FACS Cell type-specific Salinity Rice Arabidopsis Protoplast GAL4 

Notes

Acknowledgments

This work was supported by grants from the Australian Research Council (DP0770966 to M.T. and A.A.T.J.) and the U.S. National Institutes of Health (NIH grant R01 GM078279 to K.D.B.)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Aurelie Evrard
    • 1
  • Bastiaan O. R. Bargmann
    • 2
  • Kenneth D. Birnbaum
    • 2
  • Mark Tester
    • 3
  • Ute Baumann
    • 1
  • Alexander A. T. Johnson
    • 4
  1. 1.Australian Centre for Plant Functional GenomicsUniversity of AdelaideGlen OsmondAustralia
  2. 2.Center for Genomics and Systems BiologyNew York UniversityNew YorkUSA
  3. 3.Australian Centre for Plant Functional GenomicsUrrbraeAustralia
  4. 4.ACPFG and School of BotanyUniversity of MelbourneMelbourneAustralia

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