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Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-Stressed Plant Tissues

  • Petre I. Dobrev
  • Radomira Vankova
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 913)

Abstract

Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS.

Key words

Abscisic acid Auxin Cytokinin Salinity Hormone purification Hormone analysis 

Notes

Acknowledgement

This work was supported by the MEYS CR, project no. LD11073.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institute of Experimental Botany AS CRPragueCzech Republic

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