Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-Stressed Plant Tissues

  • Petre I. Dobrev
  • Radomira Vankova
Part of the Methods in Molecular Biology book series (MIMB, volume 913)


Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS.

Key words

Abscisic acid Auxin Cytokinin Salinity Hormone purification Hormone analysis 



This work was supported by the MEYS CR, project no. LD11073.


  1. 1.
    Cutler SR, Rodriguez PL, Finkelstein RR et al (2010) Abscisic acid: emergence of a core signaling network. Annu Rev Plant Biol 61:651–679PubMedCrossRefGoogle Scholar
  2. 2.
    Woodward AW, Bartel B (2005) Auxin: regulation, action, and interaction. Ann Bot 95:707–735PubMedCrossRefGoogle Scholar
  3. 3.
    Miller CO, Skoog F, Von Saltza MH et al (1955) Kinetin, a cell division factor from deoxyribonucleic acid. J Am Chem Soc 78:1345–1350Google Scholar
  4. 4.
    Dobrev P, Motyka V, Gaudinova A et al (2002) Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. Plant Physiol Biochem 40:333–337CrossRefGoogle Scholar
  5. 5.
    Chernyadev II (2009) The protective action of cytokinins on the photosynthetic machinery and productivity of plants under stress. Appl Biochem Microbiol 45:351–362CrossRefGoogle Scholar
  6. 6.
    Skoog F, Strong FM, Miller CO (1965) Cytokinins. Science 148:532–533PubMedCrossRefGoogle Scholar
  7. 7.
    Hirose N, Takei K, Kuroha T et al (2008) Regulation of cytokinin biosynthesis, compartmentation and translocation. J Exp Bot 59:75–83PubMedCrossRefGoogle Scholar
  8. 8.
    Mertens R, Deusneumann B, Weiler EW (1983) Monoclonal-antibodies for the detection and quantitation of the endogenous plant-growth regulator, abscisic acid. FEBS Lett 160:269–272CrossRefGoogle Scholar
  9. 9.
    Dobrev P, Kaminek M (2002) Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. J Chromatogr A 950:21–29PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institute of Experimental Botany AS CRPragueCzech Republic

Personalised recommendations