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Quantification of the Antioxidant Activity in Salt-Stressed Tissues

  • Jelena J. Dragišić Maksimović
  • Branka D. Živanović
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 913)

Abstract

Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu’s procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.

Key words

Ascorbate peroxidase Catalase Protein concentration Salinity Superoxide dismutase Total antioxidant capacity Total phenolic content 

Notes

Acknowledgment

This work was supported by the Serbian Ministry of Education and Science (grants 173028 and 173040).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jelena J. Dragišić Maksimović
    • 1
  • Branka D. Živanović
    • 1
  1. 1.Institute for Multidisciplinary ResearchUniversity of BelgradeBelgradeSerbia

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