The recent advances made in the use of infrared thermal imaging (thermography) as a non-invasive, high-throughput technique for the screening of salinity tolerance in plants is reviewed. Taking wheat seedlings as an example, the methods and protocols used to impose a homogeneous salt stress to a large number of genotypes, as well as capturing infrared images of these genotypes and automatically processing the images are described in detail in this chapter. We also present the source code of the Matlab program applied to automatically identify plants and batch process IR images.
Salt Wheat Barley Transpiration rate Stomatal conductance Osmotic stress
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The authors wish to thank Dr Robert Furbank for helpful comments on the manuscript and Mrs Carol Blake and Mr Scott Berry for their technical skills. Part of this research was conducted at the High Resolution Plant Phenomics Center (Canberra node of the Australian Plant Phenomics Facility).
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