Advertisement

Infrared Thermography in Plant Phenotyping for Salinity Tolerance

  • Richard A. James
  • Xavier R. R. Sirault
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 913)

Abstract

The recent advances made in the use of infrared thermal imaging (thermography) as a non-invasive, high-throughput technique for the screening of salinity tolerance in plants is reviewed. Taking wheat seedlings as an example, the methods and protocols used to impose a homogeneous salt stress to a large number of genotypes, as well as capturing infrared images of these genotypes and automatically processing the images are described in detail in this chapter. We also present the source code of the Matlab program applied to automatically identify plants and batch process IR images.

Key words

Salt Wheat Barley Transpiration rate Stomatal conductance Osmotic stress 

Notes

Acknowledgements

The authors wish to thank Dr Robert Furbank for helpful comments on the manuscript and Mrs Carol Blake and Mr Scott Berry for their technical skills. Part of this research was conducted at the High Resolution Plant Phenomics Center (Canberra node of the Australian Plant Phenomics Facility).

References

  1. 1.
    Munns R (1993) Physiological processes limiting plant growth in saline soil: some dogmas and hypotheses. Plant Cell Environ 16:15–24CrossRefGoogle Scholar
  2. 2.
    Epstein E (1980) Responses of plant to saline environments. In: Rains DW, Valentine RC, Hollaender A (eds) Genetic engineering of osmoregulation. Plenum Press, New YorkGoogle Scholar
  3. 3.
    Munns R, Schachtman DP, Condon AG (1995) The significance of a two-phase growth response to salinity in wheat and barley. Aust J Plant Physiol 22:561–569CrossRefGoogle Scholar
  4. 4.
    Munns R, James RA, Lauchli A (2006) Approaches to increasing the salt tolerance of wheat and other cereals. J Exp Bot 57:1025–1043PubMedCrossRefGoogle Scholar
  5. 5.
    Rahnama A et al (2010) Stomatal conductance as a screen for osmotic stress tolerance in durum wheat growing in saline soil. Funct Plant Biol 37:255–263CrossRefGoogle Scholar
  6. 6.
    Passioura JB, Munns R (2000) Rapid environmental changes that affect leaf water status induce transient surges or pauses in leaf expansion rate. Aust J Plant Physiol 27:941–948Google Scholar
  7. 7.
    Fricke W et al (2004) Rapid and tissue - specific changes in ABA and in growth rate in response to salinity in barley leaves. J Exp Bot 55:1115–1123PubMedCrossRefGoogle Scholar
  8. 8.
    Yeo AR, Caporn SJM, Flowers TJ (1985) The effect of salinity upon photosynthesis in rice: gas exchange by individual leaves in relation to their salt content. J Exp Bot 36:124–148Google Scholar
  9. 9.
    Sibole JV et al (1998) Role of sodium in the ABA-mediated long-term growth response of bean to salt stress. Physiol Plant 104:299–305CrossRefGoogle Scholar
  10. 10.
    Fuchs M (1990) Infrared measurement of canopy temperature and detection of plant water stress. Theor Appl Climatol 42:253–261CrossRefGoogle Scholar
  11. 11.
    Sirault XRR, James RA, Furbank RT (2009) A new screening method for osmotic component of salinity tolerance in cereals using infrared thermography. Funct Plant Biol 36:970–977CrossRefGoogle Scholar
  12. 12.
    Jones HG (1999) Use of thermography for quantitative studies of spatial and temporal variation of stomatal conductance over leaf surfaces. Plant Cell Environ 22:1043–1055CrossRefGoogle Scholar
  13. 13.
    James RA et al (2002) Factors affecting CO2 assimilation, leaf injury and growth in salt-stressed durum wheat. Funct Plant Biol 29:1393–1403CrossRefGoogle Scholar
  14. 14.
    Munns R, James RA (2003) Screening methods for salinity tolerance: a case study with tetraploid wheat. Plant Soil 253:201–218CrossRefGoogle Scholar
  15. 15.
    James RA et al (2008) Genetic variation in tolerance to the osmotic stress component of salinity stress in durum wheat. Funct Plant Biol 35:111–123CrossRefGoogle Scholar
  16. 16.
    Genc Y, McDonald GK, Tester M (2007) Reassessment of tissue Na+ concentration as a criterion for salinity tolerance in bread wheat. Plant Cell Environ 30:1486–1498PubMedCrossRefGoogle Scholar
  17. 17.
    Passioura JB (2006) The perils of pot experiments. Funct Plant Biol 33:1075–1079CrossRefGoogle Scholar
  18. 18.
    Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239–250PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.CSIRO Plant IndustryCanberraAustralia
  2. 2.High Resolution Plant Phenomics CentreCanberraAustralia

Personalised recommendations