Construction of Human Naive Antibody Gene Libraries

  • Michael Hust
  • André Frenzel
  • Torsten Meyer
  • Thomas Schirrmann
  • Stefan Dübel
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 907)

Abstract

Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies. Here, two technologies are competing the hybridoma technology using transgenic mice with human antibody gene loci and antibody phage display. The starting point for the selection of human antibodies against any target is the construction of an antibody phage display gene library.

In this review we describe the construction of human naive and immune antibody gene libraries for antibody phage display.

Key words

Antibody Antibody gene library scFv Phage display 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Michael Hust
    • 1
  • André Frenzel
    • 1
  • Torsten Meyer
    • 2
  • Thomas Schirrmann
    • 1
  • Stefan Dübel
    • 1
  1. 1.Institut für Biochemie und BiotechnologieTechische Universität BraunschweigBraunschweigGermany
  2. 2.Institute für Biochemie and BiotechnologieTechische Universität BraunschweigBraunschweigGermany

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