Antibody Engineering pp 667-679

Part of the Methods in Molecular Biology book series (MIMB, volume 907)

Selection and Use of Intracellular Antibodies (Intrabodies)

Protocol

Abstract

Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.

Key words

Phage display Intrabody Live cell imaging 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Institut Curie, CNRS UMR144ParisFrance
  2. 2.Laboratoire de Spectrométrie Physique, CNRS UMR 5588Université Joseph FourierSaint Martin d’HèresFrance

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