Selection of Antibody Fragments by Yeast Display

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 907)

Abstract

The critical need for renewable, high-quality affinity reagents in biological research, as well as for diagnostic and therapeutic applications, has required the development of new platforms of discovery. Yeast display is one of the main methods of in vitro display technology with phage display. Yeast display has been chosen by numerous groups to refine both affinity and specificity of antibodies because it enables fine discrimination between mutant clones of similar affinity. In addition, the construction of display libraries of antibody fragments in yeast permit to sample the immune antibody repertoire more fully than using phage. This chapter gives an updated overview of the available systems of yeast display platforms and libraries, followed up by technical descriptions of selection methods of antibody fragments by yeast display.

Key words

Yeast display scFv libraries pYD pAGA2 Magnetic sorting Flow sorting Panning Homologous recombination p416 BCCP 
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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Center for Research on Reproduction and Women’s Health, Penn Ovarian Cancer Research CenterUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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