Nanoparticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions

  • Matthew WrightEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 906)


Nanoparticle Tracking Analysis (NTA) provides direct and real time visualization, sizing, and counting of particulate materials between 10 nm and 1 μm in liquid suspension. The technique works on a particle-by-particle basis, relating the degree of movement under Brownian motion to the sphere equivalent hydrodynamic diameter particle size, allowing high resolution particle size distributions to be obtained within minutes. Here we describe application of NTA to the analysis of 100 nm polystyrene nanospheres in liquid suspension. The technique can be adapted for use with nearly all particulate materials with sizes between 10 nm and 1 μm.

Key words

NTA Nanoparticle tracking analysis Nanoparticles Sizing Counting Visualization NanoSight Light scattering 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.NanoSight LtdAmesburyUK

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