Nanoparticles in Biology and Medicine pp 239-252

Part of the Methods in Molecular Biology book series (MIMB, volume 906)

Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging

  • Jasmin
  • Ana Luiza Machado Torres
  • Linda Jelicks
  • Antonio Carlos Campos de Carvalho
  • David C. Spray
  • Rosalia Mendez-Otero
Protocol

Abstract

Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamagnetic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.

Key words

Stem cells Cell labeling Ferumoxides Superparamagnetic iron oxide nanoparticles Magnetic resonance imaging 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jasmin
    • 1
    • 2
  • Ana Luiza Machado Torres
    • 1
  • Linda Jelicks
    • 3
  • Antonio Carlos Campos de Carvalho
    • 1
    • 2
  • David C. Spray
    • 2
  • Rosalia Mendez-Otero
    • 1
  1. 1.Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Department of NeuroscienceAlbert Einstein College of MedicineBronxUSA
  3. 3.Department of Physiology and BiophysicsAlbert Einstein College of MedicineBronxUSA

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