Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis

  • Babu R. Prasad
  • Enda O’Connell
  • Terry J. Smith
  • Valérie A. Gérard
  • Yurii K. Gun’ko
  • Yury Rochev
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 906)

Abstract

Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.

Key words

HPMEC cells Quantum dots RNA microarray Gene expression 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Babu R. Prasad
    • 1
  • Enda O’Connell
    • 1
  • Terry J. Smith
    • 1
  • Valérie A. Gérard
    • 2
  • Yurii K. Gun’ko
    • 2
  • Yury Rochev
    • 1
  1. 1.National Centre for Biomedical Engineering ScienceNational University of IrelandGalwayIreland
  2. 2.CRANN and the School of ChemistryTrinity College DublinDublinIreland

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