Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis
Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.
Key wordsHPMEC cells Quantum dots RNA microarray Gene expression
This work was funded by the Science Foundation Ireland (SFI) and National University of Ireland Galway Millennium Fund 2008. HPMEC-ST1.6R cells were kindly provided by Ronald E. Unger, Institute of Pathology, Johannes Gutenberg University, Mainz, Germany.