Protocol

Diagnosis of Sexually Transmitted Diseases

Volume 903 of the series Methods in Molecular Biology pp 295-306

Date:

Protocol for the Detection of Treponema pallidum in Paraffin-Embedded Specimens

  • Cheng-Yen ChenAffiliated withLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention Email author 
  • , Allan PillayAffiliated withLaboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and TB Prevention, Centers for Disease Control and Prevention

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Abstract

Formalin-fixed paraffin-embedded (FFPE) tissue blocks are routinely used for histopathological examination and are also useful for specific pathogen detection by polymerase chain reaction (PCR). FFPE tissue is stable at ambient temperature for an extended period of time and relatively easy to transport compared to fresh tissue, which has to be processed or frozen immediately. In addition, archival material is an invaluable source for retrospective molecular and clinical investigation. This chapter describes detailed procedures for nucleic acid extraction and PCR detection of Treponema pallidum using FFPE tissue.

Key words

Polymerase chain reaction PCR Real-time PCR Treponema pallidum FFPE Paraffin-embedded specimen