Diagnosis of Sexually Transmitted Diseases pp 263-271

Part of the Methods in Molecular Biology book series (MIMB, volume 903)

Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer

  • Chou-Pong Pau
  • Susan K. Wells
  • Timothy C. Granade


This chapter describes a real-time PCR method for the detection of HIV-1 proviral DNA in whole blood samples using a novel double-stranded primer system. The assay utilizes a simple commercially available DNA extraction method and a rapid and easy-to-perform real-time PCR protocol to consistently detect a minimum of four copies of HIV-1 group M proviral DNA in as little as 90 min after sample (whole blood) collection. Co-amplification of the human RNase P gene serves as an internal control to monitor the efficiency of both the DNA extraction and amplification. Once the assay is validated properly, it may be suitable as an alternative confirmation test for HIV-1 infections in a variety of HIV testing venues including the mother-to-child transmission testing sites, clinics, and diagnostic testing centers.

Key words

HIV-1 proviral DNA Real-time PCR Double-stranded primer Rapid PCR Nucleic acid testing 


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Chou-Pong Pau
    • 1
  • Susan K. Wells
    • 1
  • Timothy C. Granade
    • 1
  1. 1.Laboratory Branch, Division of HIV/AIDS PreventionNational Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and PreventionAtlantaUSA

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