Protocol for the Use of Enzyme-Linked Hybridization Assays for Genital Ulcer Disease

  • Seweryn Bialasiewicz
  • Ian M. Mackay
  • David M. Whiley
  • Theo P. Sloots
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 903)

Abstract

Etiologic agents of genital ulcer disease include herpes simplex 1 and 2, Treponema pallidum pallidum, Haemophilus ducreyi, and Klebsiella granulomatis. The advent of PCR has allowed for more rapid and sensitive detection of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.

Key words

Herpes simplex Haemophilus ducreyi Treponema pallidum Klebsiella granulomatis Genital ulcer Enzyme-linked hybridization Colorimetric Multiplex PCR Detection 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Seweryn Bialasiewicz
    • 1
  • Ian M. Mackay
    • 1
  • David M. Whiley
    • 1
  • Theo P. Sloots
    • 1
  1. 1.Sir Albert Sakzewski Virus Research CentreHerstonAustralia

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