Bioengineering of Oleaginous Yeast Yarrowia lipolytica for Lycopene Production

  • Rick W. YeEmail author
  • Pamela L. Sharpe
  • Quinn Zhu
Part of the Methods in Molecular Biology book series (MIMB, volume 898)


Oleaginous yeast Yarrowia lipolytica is capable of accumulating large amount of lipids. There is a growing interest to engineer this organism to produce lipid-derived compounds for a variety of applications. In addition, biosynthesis of value-added products such as carotenoid and its derivatives have been explored. In this chapter, we describe methods to integrate genes involved in lycopene biosynthesis in Yarrowia. Each bacterial gene involved in lycopene biosynthesis, crtE, crtB, and crtI, will be assembled with yeast promoters and terminators and subsequently transformed into Yarrowia through random integration. The engineered strain can produce lycopene under lipid accumulation conditions.

Key words

Yeast Yarrowia Lipid accumulation Carotenoid Lycopene 


  1. 1.
    Bankar A, Kumar A, Zinjarde S (2009) Environmental and industrial applications of Yarrowia lipolytica. Appl Microbiol Biotechnol 84:847–865PubMedCrossRefGoogle Scholar
  2. 2.
    Zhu Q, Xue Z, Yadav N, Damude H, Pollak DW, Rupert R, Seip J, Hollerback D, Macool D, Zhang H, Bledsoe S, Short D, Tyreus B, Kinney A, Picataggio S (2010) Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. In: Cohen Z, Ratledge C (eds) Single cell oil, 2nd edn. ACOS Press, Urbana, pp 51–73Google Scholar
  3. 3.
    Sabirova J, Haddouche R, Van Bogaert I, Mulaa F, Verstraete W, Timmis K, Schmidt-Dannert C, Nicaud J, Soetaert W (2010) The “LipoYeasts” project: using the oleaginous yeast Yarrowia lipolytica in combination with specific bacterial genes for the bioconversion of lipids, fats and oils into high-value products. Microb Biotechnol 4:47–54CrossRefGoogle Scholar
  4. 4.
    Bhosale P, Bernstein P (2005) Microbial xanthophylls. Appl Microbiol Biotechnol 68:445–455PubMedCrossRefGoogle Scholar
  5. 5.
    Misawa N, Shimada H (1997) Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts. J Biotechnol 59:169–181PubMedCrossRefGoogle Scholar
  6. 6.
    Mauersberger S, Wang H, Gaillardin C, Barth G, Nicaud J (2001) Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization. J Bacteriol 183:5102–5109PubMedCrossRefGoogle Scholar
  7. 7.
    Ye R, Stead K, Yao H, He H (2006) Mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin. Appl Environ Microbiol 72:5829–5837PubMedCrossRefGoogle Scholar
  8. 8.
    O’Fallon JV, Busboom JR, Nelson ML, Gaskins CT (2007) A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. J Anim Sci 85: 1511–1521PubMedCrossRefGoogle Scholar
  9. 9.
    Yu X, Zheng Y, Dorgan KM, Chen S (2011) Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 102(10): 6134–6140PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.WilmingtonUSA
  2. 2.E.I. DuPont de Nemours, Inc.WilmingtonUSA
  3. 3.WilmingtonUSA

Personalised recommendations