MSQuant: A Platform for Stable Isotope-Based Quantitative Proteomics

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 893)

Abstract

Quantitative approaches in proteomics are emerging as a powerful tool to probe the dynamics of protein expression across biological conditions. Thereby, quantification helps to recognize proteins with potential biological relevance, which greatly aids in the design of follow-up experiments. Although multiple methods have been established that are based on stable-isotope labeling and label-free approaches, one of the remaining bottlenecks is the analysis and quantification of proteins in large datasets. MSQuant is a platform for protein quantification, capable of handling multiple labeling strategies and supporting several vendor data formats. Here, we report on the use and versatility of MSQuant.

Key words

Protein quantification Stable isotope labeling Quantitative proteomics Automation 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, Centre for High-Throughput BiologyUniversity of British ColumbiaVancouverCanada
  2. 2.Genome Biology Unit & Proteomics Core FacilityEMBLHeidelbergGermany

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