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Detection of Apoptosis by TUNEL Assay

  • Kateryna Kyrylkova
  • Sergiy Kyryachenko
  • Mark Leid
  • Chrissa Kioussi
Part of the Methods in Molecular Biology book series (MIMB, volume 887)

Abstract

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

Key words

Apoptosis TUNEL Immunofluorescence Mouse embryos Frozen sections 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Kateryna Kyrylkova
    • 1
  • Sergiy Kyryachenko
    • 1
  • Mark Leid
    • 1
  • Chrissa Kioussi
    • 2
  1. 1.Department of Pharmaceutical Sciences, College of PharmacyOregon State UniversityCorvallisUSA
  2. 2.College of Pharmacy, Department of Pharmaceutical SciencesOregon State UniversityCorvallisUSA

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