Odontogenesis pp 135-148 | Cite as

Methods to Validate Tooth-Supporting Regenerative Therapies

  • Miguel Padial-Molina
  • Julie T. Marchesan
  • Andrei D. Taut
  • Qiming Jin
  • William V. Giannobile
  • Hector F. RiosEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 887)


In humans, microbially induced inflammatory periodontal diseases are the primary initiators that disrupt the functional and structural integrity of the periodontium (i.e., the alveolar bone, the periodontal ligament, and the cementum). The reestablishment of its original structure, properties, and function constitutes a significant challenge in the development of new therapies to regenerate tooth-supporting defects. Preclinical models represent an important in vivo tool to critically evaluate and analyze the key aspects of novel regenerative therapies, including (1) safety, (2) effectiveness, (3) practicality, and (4) functional and structural stability over time. Therefore, these models provide foundational data that supports the clinical validation and the development of novel innovative regenerative periodontal technologies. Steps are provided on the use of the root fenestration animal model for the proper evaluation of periodontal outcome measures using the following parameters: descriptive histology, histomorphometry, immunostaining techniques, three-dimensional imaging, electron microscopy, gene expression analyses, and safety assessments. These methods will prepare investigators and assist them in identifying the key end points that can then be adapted to later stage human clinical trials.

Key words

Guided tissue regeneration Regenerative medicine Bone regeneration Tissue engineering Animal models Periodontal diseases Periodontal engineering 



The authors appreciate Sarah L. Volk for assisting with the preparation of the manuscript. We also acknowledge the technical contributions of Chan Ho Park, Scott Lim, and Jim Sugai. This work was supported by the NIH Grants K23DE019872 (HFR) and DE13397 (WVG). MPM was also supported by the Talentia Scholarship Program from the Regional Ministry for Innovation, Science and Enterprise, Junta de Andalusia (Spain). The authors report no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Miguel Padial-Molina
    • 1
  • Julie T. Marchesan
    • 1
  • Andrei D. Taut
    • 1
  • Qiming Jin
    • 1
  • William V. Giannobile
    • 2
  • Hector F. Rios
    • 1
    Email author
  1. 1.Department of Periodontics and Oral Medicine, School of DentistryUniversity of MichiganAnn ArborUSA
  2. 2.Michigan Center for Oral Health Research, School of DentistryUniversity of MichiganAnn ArborUSA

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