Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs
The use of deoxyoligonucleotide microarrays facilitates rapid expression profiling of gene expression using samples of about 1 μg of total RNA. Here are described practical aspects of the procedures involved, including essential reagents. Analysis of results is discussed from a practical, experimental, point of view together with software required to carry out the required statistical analysis to isolate populations of differentially expressed genes.
Key wordsmRNA Transcriptome Hybridization Fluorescence Cy3 Cy5 MA-plot Clustering .gal-file
The expert technical assistance of Mrs. Toril Woldene, Mrs. Bente Gehrken, and Mr. Benedicto Geronimo are gratefully acknowledged.
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