Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs

  • Harald Osmundsen
  • Anne-Marthe Jevnaker
  • Maria A. Landin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 887)

Abstract

The use of deoxyoligonucleotide microarrays facilitates rapid expression profiling of gene expression using samples of about 1 μg of total RNA. Here are described practical aspects of the procedures involved, including essential reagents. Analysis of results is discussed from a practical, experimental, point of view together with software required to carry out the required statistical analysis to isolate populations of differentially expressed genes.

Key words

mRNA Transcriptome Hybridization Fluorescence Cy3 Cy5 MA-plot Clustering .gal-file 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Harald Osmundsen
    • 1
  • Anne-Marthe Jevnaker
    • 1
  • Maria A. Landin
    • 1
  1. 1.Department of Oral BiologyUniversity of OsloOsloNorway

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