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Microchip Electrophoresis, with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”

  • Mohamad Reza Mohamadi
  • Romain Verpillot
  • Myriam Taverna
  • Markus Otto
  • Jean-Louis Viovy
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 869)

Abstract

Aggregation of beta amyloid peptides especially Aβ1-42 in amyloid plaques is one of the major ­neuropathological events in Alzheimer’s disease. This event is normally accompanied by a relative reduction of the concentration of Aβ1-42 in the cerebrospinal fluid (CSF) of patients developing the signs of Alzheimer’s disease. Here, we describe a microchip gel electrophoresis method in a polydimethylsiloxane (PDMS) chip that enables rapid profiling of major Aβ peptides. The method was applied to compare the relative concentration of Aβ1-42 with other Aβ peptides, for example, Aβ 1-40 in CSF. In order to increase the sensitivity of detection, Aβ peptides in the CSF samples were first captured and concentrated using magnetic beads coated with specific anti-Aβ antibodies.

Key words

Microchip electrophoresis Beta amyloid Cerebrospinal fluid and Alzheimer’s disease 

Notes

Acknowledgments

This work was supported by European grants LSHB CT 06 037953 (NeuroTAS) and FP7-NMP-09-246513 (NADINE).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mohamad Reza Mohamadi
    • 1
  • Romain Verpillot
    • 2
  • Myriam Taverna
    • 2
  • Markus Otto
    • 3
  • Jean-Louis Viovy
    • 1
  1. 1.UMR 168, Curie Institute/CNRS/Universite´ Pierre et Marie CurieParisFrance
  2. 2.University of Paris sud 11Chatenay MalabryFrance
  3. 3.Department of NeurologyUniversity of UlmUlmGermany

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