Aggregation of beta amyloid peptides especially Aβ1-42 in amyloid plaques is one of the major neuropathological events in Alzheimer’s disease. This event is normally accompanied by a relative reduction of the concentration of Aβ1-42 in the cerebrospinal fluid (CSF) of patients developing the signs of Alzheimer’s disease. Here, we describe a microchip gel electrophoresis method in a polydimethylsiloxane (PDMS) chip that enables rapid profiling of major Aβ peptides. The method was applied to compare the relative concentration of Aβ1-42 with other Aβ peptides, for example, Aβ 1-40 in CSF. In order to increase the sensitivity of detection, Aβ peptides in the CSF samples were first captured and concentrated using magnetic beads coated with specific anti-Aβ antibodies.
Microchip electrophoresis Beta amyloid Cerebrospinal fluid and Alzheimer’s disease
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This work was supported by European grants LSHB CT 06 037953 (NeuroTAS) and FP7-NMP-09-246513 (NADINE).
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