Genome-Wide Profiling of Uncapped mRNA

  • Yuling Jiao
  • José Luis Riechmann
Part of the Methods in Molecular Biology book series (MIMB, volume 876)


Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRNA synthesis and degradation. In this chapter, we describe a method to profile these uncapped mRNAs at the genome scale.

Key words

mRNA Uncapping RNA degradation Transcriptome 



The authors would like to thank Dr. E.M. Meyerowitz for his encouragement and advice. This work was supported by the US National Science Foundation 2010 Project Grant 0520193, by the National Natural Science Foundation of China Grant 31171159, and by the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.Division of Biology 156-29California Institute of TechnologyPasadenaUSA
  3. 3.Center for Research in Agricultural Genomics (CRAG)BarcelonaSpain
  4. 4.Institució Catalana de Recerca i Estudis Avançats (ICREA)BarcelonaSpain

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