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Real-Time Imaging of Tumors Using Replication-Competent Light-Emitting Microorganisms

  • Yong A. Yu
  • Stephanie Weibel
  • Aladar A. Szalay
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 872)

Abstract

Early detection of cancer and metastases is pivotal to the success of subsequent treatment intervention. In recent years, the use of live microorganisms, such as viruses and bacteria, has gained substantial research and clinical interest in both detection and therapy of cancer. Many of these live microorganisms have shown remarkable tumor-specific replication following systemic delivery. With the aid of modern molecular technologies, modified live microorganisms can be engineered to carry additional diagnostic and therapeutic capabilities. We have shown that when armed with light-emitting protein genes, such as genes for luciferase and green fluorescent protein, the entry and specific amplification of systemically-delivered vaccinia virus and bacteria in tumors can be visualized in real time using a low-light imager, or using macro- and micro-fluorescence microscopes. Therefore, through optical imaging, the location of tumors and metastases could be revealed by these light-emitting microorganisms. The tumor-colonization capability has been demonstrated in both immuno-competent as well as immuno-compromised rodent models with syngeneic and allogeneic tumors. Based on their “tumor-finding” nature, bacteria and viruses could be further designed as “vehicles” to carry multiple genes for detection and therapy of cancer.

Key words

Vaccinia virus Bacteria Systemic delivery Tumors Metastases GFP Luciferase Fluorescence and luminescence imaging 

Notes

Acknowledgements

This work was supported by an internal research grant from Genelux Corporation. The authors would like to thank Qian Zhang, Nanhai Chen, and Terry Trevino for scientific and technical contributions, and Kim Duffy for editorial assistance.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Yong A. Yu
    • 1
  • Stephanie Weibel
    • 2
  • Aladar A. Szalay
    • 3
    • 4
  1. 1.Genelux Corporation, San Diego Science CenterSan DiegoUSA
  2. 2.Department of Biochemistry, BiocenterUniversity of WuezburgWuezburgGermany
  3. 3.Rudolf-Virchow-Center, DFG-Research Center for Experimental BiomedicineUniversity of WuerzburgWuerzburgGermany
  4. 4.Genelux Corporation, San Diego Science CenterSan DiegoUSA

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