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Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells

  • Aaron M. ElliottEmail author
  • Kristi A. Hohenstein Elliott
  • Anja Kammesheidt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 873)

Abstract

During culture adaptation, human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) tend to acquire chromosomal aberrations. Generally, stem cell lines are screened for large-scale chromosomal changes using low resolution karyotype analysis. Recent studies characterizing human stem cells using array-comparative genomic hybridization (aCGH) suggests most abnormalities acquired during culture are under the resolution of karyotype analysis and therefore are routinely missed. Here, we describe a custom-designed stem cell focused microarray utilizing 44K probes, with increased resolution in relevant stem cell-associated and cancer-related genes.

Key words

Array-comparative genomic hybridization Embryonic stem cells Induced pluripotent stem cells Genome stability Karyotype 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Aaron M. Elliott
    • 1
    Email author
  • Kristi A. Hohenstein Elliott
    • 1
  • Anja Kammesheidt
    • 1
  1. 1.Ambry GeneticsAliso ViejoUSA

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