Backcross Populations and Near Isogenic Lines

  • Rik Kooke
  • Erik Wijnker
  • Joost J. B. Keurentjes
Part of the Methods in Molecular Biology book series (MIMB, volume 871)


The development of near isogenic lines (NILs) through repeated backcrossing of genetically distinct parental lines is rather straightforward. Nonetheless, depending on the available resources and the purpose of the lines to be generated, several choices can be made to guide the design of such inbred populations. Here we outline the implications of these choices and provide recommendations for the efficient and proper development of NILs for a number of common scenarios.

Key words

Near isogenic lines Chromosome substitution strains Heterogeneous inbred families Bulk segregant analysis Marker-assisted selection Genetic mapping 


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Copyright information

© Springer Science+Business Media 2012

Authors and Affiliations

  • Rik Kooke
    • 1
  • Erik Wijnker
    • 2
  • Joost J. B. Keurentjes
    • 1
    • 2
  1. 1.Laboratory of Plant PhysiologyWageningen UniversityWageningenThe Netherlands
  2. 2.Laboratory of GeneticsWageningen UniversityWageningenThe Netherlands

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