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Optimizing Saccharomyces cerevisiae Induction Regimes

  • David Drew
  • Hyun KimEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 866)

Abstract

Recombinant membrane protein yields can be optimized in Saccharomyces cerevisiae by adjusting the induction time and temperature and/or by the addition of chemical chaperones. Here we describe a protocol for assessing the importance of these parameters.

Key words

Membrane protein Overproduction Saccharomyces cerevisiae 

Abbreviations

GAL

Galactokinase

GFP

Green fluorescent protein

GPCRs

G protein-coupled receptors

TEF

Translation elongation factor 1α

Notes

Acknowledgments

This work was supported by the Royal Society (United Kingdom) through a University Research Fellowship to DD and by a Basic Science Research Program grant through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF0409-20100093) to HK.

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Copyright information

© Springer Science+business Media, LLC 2012

Authors and Affiliations

  1. 1.Division of Molecular BiosciencesMembrane Protein Crystallography Group, Imperial CollegeLondonUK
  2. 2.Laboratory of Membrane Biology, School of Biological SciencesSeoul National UniversitySeoulSouth Korea

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