Optimizing Saccharomyces cerevisiae Induction Regimes
Recombinant membrane protein yields can be optimized in Saccharomyces cerevisiae by adjusting the induction time and temperature and/or by the addition of chemical chaperones. Here we describe a protocol for assessing the importance of these parameters.
Key wordsMembrane protein Overproduction Saccharomyces cerevisiae
Green fluorescent protein
G protein-coupled receptors
Translation elongation factor 1α
This work was supported by the Royal Society (United Kingdom) through a University Research Fellowship to DD and by a Basic Science Research Program grant through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF0409-20100093) to HK.
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