Optimising Yeast as a Host for Recombinant Protein Production (Review)

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 866)

Abstract

Having access to suitably stable, functional recombinant protein samples underpins diverse academic and industrial research efforts to understand the workings of the cell in health and disease. Synthesising a protein in recombinant host cells typically allows the isolation of the pure protein in quantities much higher than those found in the protein’s native source. Yeast is a popular host as it is a eukaryote with similar synthetic machinery to the native human source cells of many proteins of interest, while also being quick, easy, and cheap to grow and process. Even in these cells the production of some proteins can be plagued by low functional yields. We have identified molecular mechanisms and culture parameters underpinning high yields and have consolidated our findings to engineer improved yeast cell factories. In this chapter, we provide an overview of the opportunities available to improve yeast as a host system for recombinant protein production.

Key words

Recombinant protein production Yeast Strain engineering Bioprocess control 

Notes

Acknowledgements

This work was supported by the European Commission via contract LSHG-CT-2004-504601 (E-MeP), LSHG-CT-2006-037793 (OptiCryst) and Grant 201924 (EDICT) to RMB. The BBSRC supports bioreactors and a flow microcalorimeter in the RMB Laboratory through an REI award.

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Copyright information

© Springer Science+business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Chemical and Biological EngineeringChalmers University of TechnologyGothenburgSweden
  2. 2.School of Life & Health Sciences and Aston Research Centre for Healthy AgeingAston UniversityBirminghamUK

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