Computer-Designed Nano-Fibrous Scaffolds
Nano-fibrous scaffolding mimics aspects of the extracellular matrix to improve cell function and tissue formation. Although several methods exist to fabricate nano-fibrous scaffolds, the combination of phase separation with reverse solid freeform fabrication (SFF) allows for scaffolds with features at three different orders of magnitude to be formed, which is not easily achieved with other nano-fiber fabrication methods. This technique allows for the external shape and internal pore structure to be precisely controlled in an easily repeatable manner, while the nano-fibrous wall architecture facilitates cellular attachment, proliferation, and differentiation of the cells. In this chapter, we examine the fabrication of computer-designed nano-fibrous scaffolds utilizing thermally induced phase separation and reverse SFF, and the benefits of such scaffolds over more traditional tissue engineering scaffolds on cellular function and tissue regeneration.
Key wordsNano-fibers Bone tissue engineering Scaffolds Solid freeform fabrication Phase separation
- 16.Wei G, Ma PX (2006) Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres. J Biomed Mater Res A 78:306–315Google Scholar
- 21.Ma PX, Langer R (1999) Fabrication of biodegradable polymer foams for cell transplantation and tissue engineering. In: Morgan J, Yarmush M (eds) Tissue engineering methods and protocols. Humana Press Inc, Totowa, NJ, pp 47–56Google Scholar
- 24.Mattioli-Belmonte M, Vozzi G, Kyriakidou K et al (2008) Rapid-prototyped and salt-leached PLGA scaffolds condition cell morpho-functional behavior. J Biomed Mater Res A 85:466–476Google Scholar
- 25.Smith LA, Beck J, Ma PX (2007) Fabrication and tissue formation with nano-fibrous scaffolds. In: Kumar C (ed) Nanotechnologies for tissue, cell and organ engineering. Wiley-VCH, Weinheim, GermanyGoogle Scholar