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Separation of Natural Products by Countercurrent Chromatography

  • James B. McAlpine
  • J. Brent Friesen
  • Guido F. Pauli
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 864)

Abstract

Countercurrent Chromatography (CCC) provides the natural product chemist with a high-resolution separatory method, which is uniquely applicable to sensitive (unstable) compounds and which allows virtually quantitative recovery of the load sample. Different instruments use different means of retaining a stationary liquid phase. The solvent system (SS) can be chosen to optimize the separatory power and the number of systems available is limitless. Several examples are provided to illustrate the power of the method and to guide the chemist in choice of an appropriate SS.

Key words

High Speed Countercurrent Chromatography (HSCCC) Centrifugal partition chromato-graphy Ito’s coil planet centrifuge pH zone refining GUESS method Two-phase solvent systems 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • James B. McAlpine
    • 1
  • J. Brent Friesen
    • 1
  • Guido F. Pauli
    • 1
  1. 1.Department of Medicinal Chemistry and Pharmacognosy, School of PharmacyUniversity of Illinois at ChicagoChicagoUSA

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