Extraction and Isolation of Saponins

  • Runner R. T. MajindaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 864)


Due to their special structural features, extraction and isolation of saponins poses a serious challenge. Conventional methods have been explored as well as the recent, relatively greener, efficient, solvent-economic, time-saving, newer methods of extraction. Both traditional and recent methods of isolation are also discussed. Finally, examples are given involving both conventional and newer methods of extraction and isolation. Though in general it is difficult to use a single technique for isolation of saponins, recent literature work seems to point to the fact that high speed counter-current separation coupled to evaporative light scattering detector (ELSD) gives superior separation. The ELSD appears to have circumvented the long-standing problem of saponin detection as most of these do not have a chromophore, and hence making UV detection only nonspecific and at range 200–210 nm.

Key words

Saponins Soap-like (mono, bi, tri)-Desmosidic Microwave-assisted extraction Ultrasound-assisted extractions HPLC HSCCC ELSD 


  1. 1.
    Hostettmann K, Marston A (1995) Saponins. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  2. 2.
    Çalis I, Yusofoglu H, Zerbe O, Sticher O (1999) Cephalotoside A: a tridesmosidic cycloartane type glycoside from Astralagus cephalotes var. brevecalys. Phytochemistry 50:843–847CrossRefGoogle Scholar
  3. 3.
    Yesilada E, Bedir E, Çalis I, Takaishi Y, Ohmoto Y (2005) Effects of triterpene saponins from Astralagus species on in vitro cytokine release. J Ethnopharmacol 96:71–77PubMedCrossRefGoogle Scholar
  4. 4.
    Semmar N, Tomofumi M, Mrabet Y, Lacaille-Dubois M-A (2010) Two new acylated tridesmosidic saponins from Astralagus armatus. Helv Acta Chim 93:871–876Google Scholar
  5. 5.
    Kuljanabhagavad T, Wink M (2009) Biological activities and chemistry of saponins from Chenopodium quinoa Willd. Phytochem Rev 8:473–490CrossRefGoogle Scholar
  6. 6.
    Inose Y, Miyase T, Ueno A (1992) Studies on constituents of Solidago virga-aurea L. II. Structures of solidagosaponins. Chem Pharm Bull 40:946–953CrossRefGoogle Scholar
  7. 7.
    Vincken J-P, Heng L, de Groot A, Gruppen H (2007) Saponins, classification and occurrence in the plant kingdom. Phytochemistry 68:275–276PubMedCrossRefGoogle Scholar
  8. 8.
    Oleszek W, Bialy Z (2006) Chromatographic determination of plant saponins – an update (2002–2005). J Chromatogr A 1112:78–91PubMedCrossRefGoogle Scholar
  9. 9.
    Li B, Abliz Z, Tang M, Fu G, Yu S (2006) Rapid structural characterization of triterpenoid saponins in crude extract from Symplocos chinensis using liquid chromatography combined with electrospray ionization tandem mass spectrometry. J Chromatogr A 1101:53–62PubMedCrossRefGoogle Scholar
  10. 10.
    Ha YW, Lim SS, Ha IN, Na Y-C, Seo J-J, Shin H, Son SH, Kim YS (2007) Preparative isolation of four ginsenosides from Korean red gingseng (steam-treated Panax gingseng C. A. Meyer), by high-speed counter-current chromatography coupled with evaporative light scattering detection. J Chromatogr A 1151:37–44Google Scholar
  11. 11.
    Qi X, Ignatova S, Luo G, Liang Q, Jun FW, Wang Y, Sutherland I (2010) Preparative isolation and purification of Rf, Re, Rd and Rb1 from the roots of Panax gingseng with a salt containing solvent system and flow step-gradient by high performance counter-current chromatography with evaporative light scattering detector. J Chromatogr A 1217:1995–2001PubMedCrossRefGoogle Scholar
  12. 12.
    Müller A, Ganzera M, Stuppner H (2005) Analysis of phenolic glycosides and saponins in Primula elatior and Primula veris (primula root) by liquid chromatography, evaporative light scattering detection and mass spectrometry. J Chromatogr A 1112:218–223PubMedCrossRefGoogle Scholar
  13. 13.
    Ito Y, Conway WD (1996) High speed counter-current chromatography. Wiley-Interscience, New YorkGoogle Scholar
  14. 14.
    Du Q, Cheng H, Ito Y (2001) Separation of radioactive metabolites in cultured tea cells fed with [14C]phenylalanine using high-speed counter-current chromatography. J Chromatogr A 921:331–334PubMedCrossRefGoogle Scholar
  15. 15.
    Du QZ, Li ZH, Ito Y (2001) Preparative separation of isoflavone components in soybeans using high-speed counter-current chromatography. J Chromatogr A 921:271–274CrossRefGoogle Scholar
  16. 16.
    Du Q, Xia M, Ito Y (2002) Purification of icariin from the extract of Epimedium segittatum using high-speed counter-current chromatography. J Chromatogr A 962:239–241PubMedCrossRefGoogle Scholar
  17. 17.
    Du QZ, Chai WJ, Ito Y (2002) Preparative separation of fruit extract of Silybum marianum using a high-speed countercurrent chromatograph with scale-up columns. J Liq Chromatogr Rel Technol 25:2515–2520CrossRefGoogle Scholar
  18. 18.
    Weng A, Jenett-Siems K, Schmieder P, Bachran D, Bachran C, Görick C, Thakur M, Fuchs H, Melzig MF (2010) A convenient method for saponin isolation in tumor therapy. J Chromatogr B 878:713–718CrossRefGoogle Scholar
  19. 19.
    Chen Y, Xie M-Y, Gong X-F (2007) Microwave-assisted extraction used for the isolation of total triterpenoid saponins form Ganoderma atrum. J Food Eng 81:162–170CrossRefGoogle Scholar
  20. 20.
    Kwon JH, Bélanger JMR, Páre JRJ (2003) Optimisation of microwave-assisted extraction (MAE) for gingseng components by response surface methodology. J Agric Food Chem 51:1807–1810PubMedCrossRefGoogle Scholar
  21. 21.
    Kwon JH, Bélanger JMR, Páre JRJ, Yaylayan VA (2003) Applications of microwave-assisted process (MAP™) to the fast extraction of ginseng saponins. Food Res Int 36:491–498CrossRefGoogle Scholar
  22. 22.
    Kwon JH, Lee GD, Bélanger JMR, Páre JRJ (2003) Effects of ethanol concentration on the efficiency of extraction of ginseng saponins when using when using a microwave-assisted process (MAP™). Int J Food Sci Tech 38:615–622CrossRefGoogle Scholar
  23. 23.
    Vongsangnak W, Gua J, Chauvatcharin S, Zhong JJ (2004) Towards efficient extraction of notoginseng saponins from cultured cells of Panax notoginseng. Biochem Eng J 18:115–120CrossRefGoogle Scholar
  24. 24.
    Wu J, Lin L, Chau F (2001) Ultrasound-assisted extraction of ginseng spaonins cultured in ginseng cells. Sonochem 8:347–352CrossRefGoogle Scholar
  25. 25.
    Kerem Z, German-Shashoua H, Yaden O (2005) Microwave-assisted extraction of bioactive saponins from chickpea (Cicer arietinum L). J Sci Food Agric 85:406–412CrossRefGoogle Scholar
  26. 26.
    Eskilsson CS, Bjorkland E (2000) Analytical-scale microwave-assisted extraction. J Chromatogr A 902:227–250PubMedCrossRefGoogle Scholar
  27. 27.
    Marston A, Wolfender JL, Hostettmann K (2000) Analysis and isolation of saponins from plant material. In: Oleszek WA, Marston A (eds) Saponins in foodstuffs and medicinal plants. Annual proceedings of the phytochemical society, Clarendon, pp 1–12Google Scholar
  28. 28.
    Oleszek WA (2002) Chromatographic determination of plant saponins. J Chromatogr A 967:147–162PubMedCrossRefGoogle Scholar
  29. 29.
    Woldemichael GM, Wink M (2001) Identification and biological activities of triterpenoid saponins from Chenopodium quinoa. J Agric Food Chem 49:2327–2332PubMedCrossRefGoogle Scholar
  30. 30.
    Kuljanabhagavad T, Thongphasuk P, Chamulitrat W, Wink M (2008) Triterpene saponins from Chenopodium quinoa Willd. Phytochemistry 69:1919–1926PubMedCrossRefGoogle Scholar
  31. 31.
    Schöpke T, Agha MIH, Wray V, Hiller K (1994) Triterpenoid saponins from Bellium bellidioides. Phytochemistry 36:449–453PubMedCrossRefGoogle Scholar
  32. 32.
    Schöpke T, Wray V, Nimtz M, Hiller K (1994) Triterpenoid saponins from Bellium bellidioides. Structure of the minor deacylsaponins. Phytochemistry 41:1399–1403CrossRefGoogle Scholar
  33. 33.
    Miyase T, Shiokawa K-I, Zhang DM, Ueno A (1996) Araliasaponin I–XI, triterpene saponins from the roots of Aralia decaisneana. Phytochemistry 41:1411–1418PubMedCrossRefGoogle Scholar
  34. 34.
    Du Q, Jerz G, Waibel R, Winterhalter P (2003) Isolation of dammarane saponins from Panax ginseng by high speed counter-current chromatography. J Chromatogr A 1008:173–180PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of BotswanaGaboroneBotswana

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