Difference Gel Electrophoresis (DIGE) pp 3-8

Part of the Methods in Molecular Biology book series (MIMB, volume 854)

DIGE: Past and Future

Protocol

Abstract

This chapter provides a brief historical perspective of the development of difference gel electrophoresis, from its inception to commercialization and beyond.

Key words

Difference gel electrophoresis Historical perspective 

References

  1. 1.
    Ünlü, M., Morgan, M. E., and Minden, J. S. (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts, Electrophoresis 18, 2071–2077.PubMedCrossRefGoogle Scholar
  2. 2.
    Helling, S., Schmitt, E., Joppich, C., Schulenborg, T., Mullner, S., Felske-Muller, S., Wiebringhaus, T., Becker, G., Linsenmann, G., Sitek, B., Lutter, P., Meyer, H. E., and Marcus, K. (2006) 2-D differential membrane proteome analysis of scarce protein samples, Proteomics 6, 4506–4513.PubMedCrossRefGoogle Scholar
  3. 3.
    Comunale, M. A., Mattu, T. S., Lowman, M. A., Evans, A. A., London, W. T., Semmes, O. J., Ward, M., Drake, R., Romano, P. R., Steel, L. F., Block, T. M., and Mehta, A. (2004) Comparative proteomic analysis of de-N-glycosylated serum from hepatitis B carriers reveals polypeptides that correlate with disease status, Proteomics 4, 826–838.PubMedCrossRefGoogle Scholar
  4. 4.
    Ruan, Y., and Wan, M. (2007) An optimized procedure for solubilization, reduction, and transfer of human breast cancer membrane-enriched fraction by 2-DE, Electrophoresis 28, 3333–3340.PubMedCrossRefGoogle Scholar
  5. 5.
    Han, M. J., Herlyn, M., Fisher, A. B., and Speicher, D. W. (2008) Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts, Electrophoresis 29, 695–705.PubMedCrossRefGoogle Scholar
  6. 6.
    Sitek, B., Sipos, B., Pfeiffer, K., Grzendowski, M., Poschmann, G., Hawranke, E., Koper, K., Kloppel, G., Meyer, H. E., and Stuhler, K. (2008) Establishment of “one-piece” large-gel 2-DE for high-resolution analysis of small amounts of sample using difference gel electrophoresis saturation labelling, Anal Bioanal Chem 391, 361–365.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biological SciencesCarnegie Mellon UniversityPittsburghUSA

Personalised recommendations