Single-Cell Analysis pp 13-15

Part of the Methods in Molecular Biology book series (MIMB, volume 853) | Cite as

Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview



Flow cytometry and microscopy are perhaps the two most commonly used techniques for analyzing single cells. Both techniques are typically robust and provide a high throughput analysis of living and/or fixed cells. The techniques are often combined with fluorescent labeling of cells, using for example antibodies. This chapter is a short introductory review where some of the possible applications using flow cytometry and microscopy are discussed.

Key words

Flow cytometry Microscopy Single cells High throughput 


  1. 1.
    Givan A (2001) Flow cytometry first principles. Wiley-Liss second edition.Google Scholar
  2. 2.
    Andersson Svahn H, Van den Berg, A. (2007) Single cells or large populations? Lab on a chip 7(5):544–546.CrossRefGoogle Scholar
  3. 3.
    Mettetal JT, Muzzey D, Pedraza JM, Ozbudak EM, & van Oudenaarden A (2006) Predicting stochastic gene expression dynamics in single cells. Proceedings of the National Academy of Sciences of the United States of America 103(19):7304–7309.PubMedCrossRefGoogle Scholar
  4. 4.
    Yu J, Xiao J, Ren X, Lao K, & Xie XS (2006) Probing gene expression in live cells, one protein molecule at a time. Science 311(5767):1600–1603.PubMedCrossRefGoogle Scholar
  5. 5.
    Villas BH (1998) Flow cytometry: an overview. Cell Vis 5(1):56–61.PubMedGoogle Scholar
  6. 6.
    Krutzik PO & Nolan GP (2006) Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nature methods 3(5):361–368.PubMedCrossRefGoogle Scholar
  7. 7.
    Nolan JP & Sklar LA (1998) The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions. Nature biotechnology 16(7):633–638.PubMedCrossRefGoogle Scholar
  8. 8.
    Martin JC & Swartzendruber DE (1980) Time: a new parameter for kinetic measurements in flow cytometry. Science 207(4427):199–201.PubMedCrossRefGoogle Scholar
  9. 9.
    Roostalu J, Joers A, Luidalepp H, Kaldalu N, & Tenson T (2008) Cell division in Escherichia coli cultures monitored at single cell resolution. BMC microbiology 8:68.PubMedCrossRefGoogle Scholar
  10. 10.
    Oheim M (2007) High-throughput microscopy must re-invent the microscope rather than speed up its functions. British journal of pharmacology 152(1):1–4.PubMedCrossRefGoogle Scholar
  11. 11.
    Pepperkok R & Ellenberg J (2006) High-throughput fluorescence microscopy for systems biology. Nat Rev Mol Cell Biol 7(9):690–696.PubMedCrossRefGoogle Scholar
  12. 12.
    Harnett MM (2007) Laser scanning cytometry: understanding the immune system in situ. Nat Rev Immunol 7(11):897–904.PubMedCrossRefGoogle Scholar
  13. 13.
    Arcibal IG, Santillo MF, & Ewing AG (2007) Recent advances in capillary electrophoretic analysis of individual cells. Anal Bioanal Chem 387(1):51–57.PubMedCrossRefGoogle Scholar
  14. 14.
    Kehr J (2003) Single cell technology. Current opinion in plant biology 6(6):617–621.PubMedCrossRefGoogle Scholar
  15. 15.
    Sims CE & Allbritton NL (2007) Analysis of single mammalian cells on-chip. Lab on a chip 7(4):423–440.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Cell and Molecular Biology, Science For Life LaboratoryKarolinska InstituteStockholmSweden

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