Gene Synthesis pp 235-247 | Cite as

Design-A-Gene with GeneDesign

  • Sarah M. Richardson
  • Steffi Liu
  • Jef D. Boeke
  • Joel S. Bader
Part of the Methods in Molecular Biology book series (MIMB, volume 852)


The manual design of synthetic genes is a tedious and error-prone process—even for very short genes—and it becomes completely infeasible when multiple synthetic genes are needed. GeneDesign is a set of modules that automate batch nucleotide manipulation. Here, we explain the installation, configuration, and use of GeneDesign as part of a synthetic design workflow.

Key words

Synthetic biology Computer-assisted GeneDesign Codon optimization Synthetic genes Synthetic biology software 


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Copyright information

© Springer Sceince+Business Media, LLC 2012

Authors and Affiliations

  • Sarah M. Richardson
    • 1
  • Steffi Liu
    • 2
  • Jef D. Boeke
    • 3
  • Joel S. Bader
    • 4
    • 5
  1. 1.High Throughput Biology CenterJohns Hopkins University School of Public HealthBaltimoreUSA
  2. 2.Department of Biomedical EngineeringJohns Hopkins UniversityBaltimoreUSA
  3. 3.Department of Molecular Biology and Genetics, High Throughput Biology CenterJohns Hopkins University School of MedicineBaltimoreUSA
  4. 4.High Throughput Biology CenterJohns Hopkins University School of MedicineBaltimoreUSA
  5. 5.Whiting School of EngineeringJohns Hopkins UniversityBaltimoreUSA

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