Tissue Processing Prior to Analysis of Alzheimer’s Disease Associated Proteins and Metabolites, Including Aβ

  • Stephen D. Schmidt
  • Ralph A. Nixon
  • Paul M. Mathews
Part of the Methods in Molecular Biology book series (MIMB, volume 849)

Abstract

Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate multiple characterizations can be done, including western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELISA quantitation of Aβ in the brain of a transgenic mouse model of β-amyloid deposition. The ELISA technique is described in detail in Chapter 34.

Key words

Aβ Alzheimer’s disease Amyloid Amyloid precursor protein APP Brain Dissection Fractionation Homogenization Membrane protein SDS-PAGE Western blot analysis 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Stephen D. Schmidt
    • 1
    • 2
  • Ralph A. Nixon
    • 1
    • 3
  • Paul M. Mathews
    • 1
    • 4
  1. 1.Center for Dementia ResearchNathan S. Kline Institute for Psychiatric ResearchOrangeburgUSA
  2. 2.Vaccine Research CenterNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA
  3. 3.Departments of Psychiatry and Cell BiologyNew York University School of MedicineNew YorkUSA
  4. 4.Department of PsychiatryNew York University School of MedicineNew YorkUSA

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