Histological Staining of Amyloid and Pre-amyloid Peptides and Proteins in Mouse Tissue
The increased availability of transgenic mouse models for studying human diseases has shifted the focus of many laboratories from in vitro to in vivo assays. Herein, methods are described to allow investigators to obtain well-preserved mouse tissue to be stained with the standard histological dyes for amyloid, Congo Red, and Thioflavin S. These sections can as well be used for immunohistological procedures that allow detection of tissue amyloid and pre-amyloid, such as those composed of the amyloid-β peptide, the tau protein, and the islet amyloid polypeptide.
Key wordsMouse Perfusion Brain Pancreas Fixation Histology Congo red Thioflavin S Immunohistochemistry Amyloid-β Plaques Tau Tangles Islet amyloid polypeptide
This work was supported by NIHs grant AG20197, AG032611, and DK075494 and the Alzheimer’s Association. These protocols were in part adapted from methods obtained from Stanley A. Lorens and Debra Magnuson at Loyola University Chicago. We thank Drs. Fernando Goñi and Ayodeji Asuni for their comments on the original manuscript, which has now been substantially updated and expanded in this second edition of the book.
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