Ribozymes pp 439-454 | Cite as

Rational Design and Tuning of Ribozyme-Based Devices

  • Joe C. Liang
  • Christina D. SmolkeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 848)


A synthetic gene-regulatory device platform was described by modularly assembling three RNA components encoding distinct functions of sensing, transmission, and actuation. The molecular binding at the sensor component is translated by the transmitter component through a strand-displacement event to modulate activity of the actuator component, which then interacts with cellular transcriptional machinery to affect gene expression levels. Here, we provide some general guidelines on linking RNA components to construct gene-regulatory devices and strategies to tune device regulatory activities through an RNA folding ­program for specific cellular applications.

Key words

Rational design Ribozyme RNA folding RNA switch Synthetic biology 



The Smolke Laboratory is supported by funds from the NIH, NSF, and the Alfred P. Sloan Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Division of Chemistry and Chemical EngineeringCalifornia Institute of TechnologyPasadenaUSA
  2. 2.Bioengineering DepartmentStanford UniversityStanfordUSA

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